dna loading buffer (Thermo Fisher)

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Thermo Fisher dna loading buffer
Dna Loading Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+loading+buffer/Agarose+Gel+Loading+Dye/10__1016_slash_j__apsb__2026__06__004-84-24-27
Average 96 stars, based on 1 article reviews

dna loading buffer - by Bioz Stars, 2026-10

96/100 stars

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Article Title: NanoCRISPR-assisted biomimetic tissue-equivalent patch regenerates the intervertebral disc by inhibiting endothelial-to-mesenchymal transition.
Article Snippet: The integrity of the intervertebral disc (IVD), an immune-privileged organ protected by the blood–disc barrier, is compromised following annulus fibrosus (AF) injury.. This breach facilitates angiogenesis, immune cell infiltration, and inflammation, accelerating intervertebral disc degeneration (IDD) and resulting in various clinical disorders.. Current treatments fail to adequately address biological repair of AF defects and angiogenesis.

Amplification:

Article Title: Methylation status of PAX1 and SF-1: implications for diagnosis and prognosis in endometrial cancer
Article Snippet: .. Amplification products (20 μL) were mixed with 6× DNA Loading Buffer and analyzed by agarose gel electrophoresis with a 10 μL DNA ladder using the E-Gel Imager Gel Imaging System (Thermo Fisher, USA). ..

Agarose Gel Electrophoresis:

Article Title: Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs
Article Snippet: Plasmid DNA for titration experiments was quantified using a Qubit fluorometer (Invitrogen). .. Reactions were quenched with DNA loading buffer (30% (v/v) glycerol, 0.25% (w/v) bromophenol blue, 0.25% (w/v) xylene cyanol) containing 15 mM EDTA and separated by 1.5% agarose gel pre-stained with SYBER Gold (Invitrogen). .. For radiolabeled cleavage assays, the substrates used were 5′-end-labeled with T4 PNK (NEB) in the presence of gamma 32P-ATP.

Article Title: DNA Barcoding and Phylogenetic Relationship of Parabuthus liosoma (Ehrenberg, 1828) (Scorpiones: Buthidae) in Saudi Arabia
Article Snippet: Genomic DNA was extracted and collected from leg tissue of preserved specimens using the commercially available DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol. .. To assess DNA quality, 5 μL of genomic DNA was mixed with 1 μL of 6× DNA loading buffer (containing 0.25% w/v bromophenol blue and 40% w/v sucrose) and loaded onto a 1% agarose gel stained with 0.5 μg/mL ethidium bromide (Thermo Fisher Scientific, Waltham, MA, USA). ..

Article Title: DNA Barcoding and Phylogenetic Relationship of Parabuthus liosoma (Ehrenberg, 1828) (Scorpiones: Buthidae) in Saudi Arabia.
Article Snippet: Genomic DNA was extracted and collected from leg tissue of preserved specimens using the commercially available DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol. .. To assess DNA quality, 5 μL of genomic DNA was mixed with 1 μL of 6× DNA loading buffer (containing 0.25% w/v bromophenol blue and 40% w/v sucrose) and loaded onto a 1% agarose gel stained with 0.5 μg/mL ethidium bromide (Thermo Fisher Scientific, Waltham, MA, USA). ..

Article Title: A tumor-targeted MOF nanoplatform for synergistic ferroptosis-chemotherapy via cascade-activated enzyme-chlormethine prodrug.
Article Snippet: H2O2-activated prodrugs have been developed to alleviate the severe side effects of chemotherapy.. However, achieving precise and efficient delivery, along with high activation and release efficiency at the target site, remains a significant challenge.. In this study, we combined a H2O2-generating enzyme (glucose oxidase, GOx) with a H2O2-sensitive chlormethine prodrug via ‘‘reversible click’’ chemistry between amino groups of the enzyme and phenyl boronic acid groups of the prodrug.

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Polymerase Chain Reaction:

Article Title: Novel circUnc79 regulates morphine-induced reward memory via the miR-149-3p/EAAT2 axis and glutamatergic plasticity
Article Snippet: PCR amplification was performed using 2 × Phusion High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA, USA) with cDNA or genomic DNA as templates under standard conditions. .. PCR products were resolved on 2% agarose gels containing Ultra GelRed (Vazyme Biotech, Nanjing, China) in 1 × TAE buffer, mixed with 6 × DNA loading buffer (Thermo Fisher Scientific, Waltham, MA, USA), and separated alongside a DNA marker. .. Electrophoresis was conducted at 100 V for 30 min, and gel images were captured using a ChemiDoc XRS+ imaging system (Bio-Rad, Hercules, CA, USA) and analyzed with Image Lab software.

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